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Immunoprecipitation and immunoblotting Extraction of proteins from cultured cells was performed with a modified lysis buffer (50 mM Tris-HCl [pH 7.5], 0.1% SDS, 1% Triton X-100, 150 mM NaCl, 1 mM dithiothreitol, 0.5 mM EDTA, 100 μM PMSF, 100 μM leupeptin)

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In particular, the ferritin-like protein Dps is critical for withstanding oxidative stress, as it both sequesters iron and binds DNA to protect it from damage (Halsey et al., 2004